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RAD18-BRCTx interaction is required for efficient repair of UV-induced DNA damage

时间:2012年02月01日 访问次数:1342

DNA Repair (Amst). 2012 Feb 1;11(2):131-8.

Ting Liu, Hongxia Chen, Hongtai Kim, Michael S.Y. Huen, Junjie Chen*, Jun Huang*

Abstract

BRCA1 carboxyl-terminal (BRCT) motifs are present in a number of proteins involved in DNA repair and/or DNA damage signaling pathways. The BRCT domain-containing protein BRCTx has been shown to interact physically with RAD18, an E3 ligase involved in postreplication repair and homologous recombination repair. However, the physiological relevance of the interaction between RAD18 and BRCTx is largely unknown. In this study, we showed that RAD18 interacts with BRCTx in a phosphorylation-dependent manner and that this interaction, mediated via highly conserved serine residues on the RAD18 C terminus, is required for BRCTx accumulation at DNA damage sites. Furthermore, we uncovered critical roles of the RAD18-BRCTx module in UV-induced DNA damage repair but not PCNA mono-ubiquitination or homologous recombination. Thus, our results suggest that RAD18 has an additional function in the surveillance of the UV-induced DNA damage response signal.

全文链接:http://www.sciencedirect.com/science/article/pii/S1568786411003077