The research of Yang lab is aimed at developing new proteomic analysis techniques based on biomass spectrometry, chemical biology, and computational proteomics for studying protein-protein interactions and post-translational modification of proteins. Our specific research contents include the following aspects:
Develop probes for capturing weak and transient protein-protein interactions:
Develop efficient, low-toxicity and biomacromolecular probes to capture weak, transient protein-protein interactions in living cells, especially those interactions between enzymes and substrates. High-throughput identification of direct substrates for proteases. The use of small molecular probes to study the dynamic structure of large protein complexes, as well as the process and mechanism of protein complexes.
Develop algorithms and software for MS data analysis:
Aim at the special needs of biological mass spectrometry data analysis, we develop new software of special analysis algorithms and processes. Protein samples processed by special small molecules have long been constrained by the software's analytical capabilities in mass spectrometry, and they cannot fully utilize the application value of these small molecules in life sciences. In particular, after such samples are non-specifically digested, the existing software systems are almost powerless. The development of efficient data analysis algorithms and processes will be a research direction of our long-term efforts.