报告题目：RNA Modification by RNA-guided Enzymes

报告人：   Keqiong Ye, Ph.D.
                 Associate Investigator
                 National Institute of Biological Sciences（NIBS）     

主持人 ： 冯新华 教授

时    间： 2012年9月13日（星期四）下午4：00

地    点： 医学院综合楼205报告厅

Abstract：

Ribosomal RNAs contain a large number of pseudouridines and 2'-O-methylated nucleotides that are introduced at specific sites after transcription. These modifications, which greatly increase the chemical diversity of rRNA, are generally beneficial and sometimes even critical for the structure and function of rRNAs. In archaeal and eukaryotic organisms, pseudouridine and 2'-O-methylated nucleotides in rRNAs are synthesized by box H/ACA and C/D RNA-protein complexes (RNPs), respectively. In each such complex, a distinct guide RNA belonging to the H/ACA or C/D RNA family determines the substrate specificity by base-pairing with the substrate around the modification site. We have analyzed the structures of H/ACA and C/D RNPs by x-ray crystallography and biochemical approaches, providing insights into their structural organization and mechanism of action.