2014年3月13日我院宋海卫课题组与黄俊课题组在Cell Reports合作发表论文:Structural Basis of SOSS1 Complex Assembly and Recognition of ssDNA

时间:2014年03月13日 访问次数:2101

Cell Rep.2014 Mar;6:1-10.

Wendan Ren, Hongxia Chen, Qiangzu Sun, Xuhua Tang, Siew Choo Lim, Jun Huang*, Haiwei Song*

Abstract

The SOSS1 complex comprising SOSSA, SOSSB1, and SOSSC senses single-stranded DNA (ssDNA) and promotes repair of DNA double-strand breaks(DSBs). But how SOSS1 is assembled and recognizes ssDNA remains elusive. The crystal structure of the N-terminal half of SOSSA (SOSSAN) in complex with SOSSB1 and SOSSC showed that SOSSAN serves as a scaffold to bind both SOSSB1 and SOSSC for assembly of the SOSS1 complex. The structures of SOSSAN/B1 in complex with a 12 nt ssDNA and SOSSAN/B1/C in complex with a 35 nt ssDNA showed that SOSSB1 interacts with both SOSSAN and ssDNA via two distinct surfaces. Recognition of ssDNA with a length of up to nine nucleotides is mediated solely by SOSSB1, whereas neither SOSSC nor SOSSAN are critical for ssDNA binding. These results reveal the structural basis of SOSS1 assembly and provide a framework for further study of the mechanism governing longer ssDNA recognition by the SOSS1 complex during DSB repair.

全文链接:http://download.cell.com/cell-reports/pdf/PIIS221112471400120X.pdf?intermediate=true